Effect of extenders and sperm concentration on migration efficiency of Malabari buck spermatozoa in bovine cervical mucus

The study evaluated the effect of extenders and sperm concentration on migration efficiency Malabari buck spermatozoa in bovine cervical mucus. Fresh semen ejaculates collected six different days from three adult healthy bucks were pooled divided into four groups based extender used (tris-egg yolk-based or liposome-based extender) rate extension (200 400 million motile per 0.5 mL) (n=6 group) subjected to cryopreservation. After freezing thawing, cryopreserved travelled significantly (p<0.05) greater distance mucus compared those preserved tris-egg when packed French medium straws with 200 progressively spermatozoa. corresponding values also showed same trend. It was observed that extended better terms both extenders.